I really think this has a lot of potential to become a great flash series. I have a few ideas for your comedic flavor.
When DK falls off the cliff a ,"Not followin' yo ass this time" would've been good, if timed right. It just felt a bit delayed.
Candy Kong getting hit on pretty hard and him saying something like "Baby why don't we go back to my place and make this, what we have goin on here, BANANAS." to her, then the rejection line : "You do know we're related, Right?" and cutting to a DK looking at the camera and his jaw slowly dropping or something.
When DK got hit by the pie in the face the dude throwing the barrels could've said something like "You were jumpin up and down so long I figured you were MONKEYING AROUND" and then laughs.
The "Owned" part. Make that bee in every single animation. Seriously. Make that bee rage like a 12 year old that just killed someone on Halo. Because you know it's the right thing to do.
Well there's my two cents and your 4 stars. Good luck sir.